Journal: Antibiotics

Article Title: Antimicrobial and Cytoprotective Effects of Tea Extracts Against Escherichia coli -Producing Colibactin Toxin Infections

doi: 10.3390/antibiotics14090886

Figure Lengend Snippet: Efficacy of tea extracts and phytochemical compounds for inhibition of transient infection with E. coli ATCC 25922 ( A ) at a MOI of 400 on Caco-2 cells for 4 h when compared to control infected epithelial cells and transient infection with E. coli K-12 ( B ).

Article Snippet: In terms of the cell cycle, the study revealed that E. coli ATCC 25922 downregulated CDK-1 and upregulated CDK-2 in Caco-2 cells, leading to cell cycle arrest and increased DNA damage.

Techniques: Inhibition, Infection, Control

Journal: Antibiotics

Article Title: Antimicrobial and Cytoprotective Effects of Tea Extracts Against Escherichia coli -Producing Colibactin Toxin Infections

doi: 10.3390/antibiotics14090886

Figure Lengend Snippet: Comet assay ( A ) and percentage of DNA damage ( B ) of Caco-2 cells infected with E. coli ATCC 25922 and treatment with tea extracts and compounds. # Tea extracts and compounds significantly reduce the DNA damage of cells from the infection compared to the E. coli ATCC 25922 infection without the treatments ( p < 0.05). Values are mean ± standard deviation; n = 3 samples. ** indicated p < 0.001. All data are used to analyze between two groups using one-way ANOVA and Tukey’s test for multiple comparisons.

Article Snippet: In terms of the cell cycle, the study revealed that E. coli ATCC 25922 downregulated CDK-1 and upregulated CDK-2 in Caco-2 cells, leading to cell cycle arrest and increased DNA damage.

Techniques: Single Cell Gel Electrophoresis, Infection, Standard Deviation

Journal: Antibiotics

Article Title: Antimicrobial and Cytoprotective Effects of Tea Extracts Against Escherichia coli -Producing Colibactin Toxin Infections

doi: 10.3390/antibiotics14090886

Figure Lengend Snippet: Expression of CDK-1, CDK-2 , and Ki-67 genes associated with the cell cycle in Caco-2 cells following infection with colibactin-producing E. coli and treatment with tea extracts and compounds. * Data are significantly different compared to cells infected with E. coli ATCC 25922 ( p < 0.05). Values are presented as mean ± standard deviation; n = 3 samples. Statistical analysis between groups was performed using one-way ANOVA and Tukey’s test for multiple comparisons.

Article Snippet: In terms of the cell cycle, the study revealed that E. coli ATCC 25922 downregulated CDK-1 and upregulated CDK-2 in Caco-2 cells, leading to cell cycle arrest and increased DNA damage.

Techniques: Expressing, Infection, Standard Deviation

Journal: Antibiotics

Article Title: Antimicrobial and Cytoprotective Effects of Tea Extracts Against Escherichia coli -Producing Colibactin Toxin Infections

doi: 10.3390/antibiotics14090886

Figure Lengend Snippet: Proposed mechanism of action by which tea extracts (green, oolong, and black tea) and tea compounds (catechin and caffeine) inhibit E. coli colibactin toxin infection in colon cells. Tea extracts and compounds showed strong antimicrobial activity against colibactin-producing E. coli . They protected Caco-2 cells from cytopathic effects (megalocytosis, cell debris), improved cell viability, and reduced colibactin-induced DNA damage. E. coli infection downregulated CDK-1 and upregulated CDK-2 , causing cell cycle arrest and DNA damage. Tea extracts and compounds restored normal cell cycle regulation (↑ CDK-1 , ↓ CDK-2 ) and catechin promoted cell proliferation via Ki-67 expression.

Article Snippet: In terms of the cell cycle, the study revealed that E. coli ATCC 25922 downregulated CDK-1 and upregulated CDK-2 in Caco-2 cells, leading to cell cycle arrest and increased DNA damage.

Techniques: Infection, Activity Assay, Expressing